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Intracellular Reactive Oxygen Species Mediate the Linkage of Na /K -ATPase to Hypertrophy and Its Marker Genes in
 

Summary: Intracellular Reactive Oxygen Species Mediate the Linkage
of Na /K -ATPase to Hypertrophy and Its Marker Genes in
Cardiac Myocytes*
(Received for publication, January 20, 1999, and in revised form, April 7, 1999)
Zijian Xie, Peter Kometiani, Jiang Liu, Jie Li, Joseph I. Shapiro, and Amir Askari
From the Departments of Pharmacology and Medicine, Medical College of Ohio, Toledo, Ohio 43614
We showed before that in cardiac myocytes partial
inhibition of Na /K -ATPase by nontoxic concentra-
tions of ouabain causes hypertrophy and transcrip-
tional regulations of growth-related marker genes
through multiple Ca2
-dependent signal pathways
many of which involve Ras and p42/44 mitogen-acti-
vated protein kinases. The aim of this work was to ex-
plore the roles of intracellular reactive oxygen species
(ROS) in these ouabain-initiated pathways. Ouabain
caused a rapid generation of ROS within the myocytes
that was prevented by preexposure of cells to N-acetyl-
cysteine (NAC) or vitamin E. These antioxidants also
blocked or attenuated the following actions of ouabain:

  

Source: Askari, Amir - Department of Biochemistry & Cancer Biology, University of Toledo
Brand, Paul H. - Department of Physiology and Pharmacology, University of Toledo

 

Collections: Biology and Medicine