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Detailed map of a cis-regulatory input function , A. E. Mayo*
 

Summary: Detailed map of a cis-regulatory input function
Y. Setty*
, A. E. Mayo*
, M. G. Surette
, and U. Alon*
Departments of *Molecular Cell Biology and Physics of Complex Systems, The Weizmann Institute of Science, Rehovot 76100, Israel; and Department of
Microbiology and Infectious Diseases, University of Calgary, Calgary, AB, Canada T2N 4N1
Edited by Curtis G. Callan, Jr., Princeton University, Princeton, NJ, and approved April 24, 2003 (received for review February 9, 2003)
Most genes are regulated by multiple transcription factors that bind
specific sites in DNA regulatory regions. These cis-regulatory regions
perform a computation: the rate of transcription is a function of the
active concentrations of each of the input transcription factors. Here,
we used accurate gene expression measurements from living cell
cultures, bearing GFP reporters, to map in detail the input function of
the classic lacZYA operon of Escherichia coli, as a function of about a
hundred combinations of its two inducers, cAMP and isopropyl
-D-thiogalactoside (IPTG). We found an unexpectedly intricate func-
tion with four plateau levels and four thresholds. This result compares
well with a mathematical model of the binding of the regulatory
proteins cAMP receptor protein (CRP) and LacI to the lac regulatory

  

Source: Alon, Uri - Departments of Molecular Cell Biology & Physics of Complex Systems, Weizmann Institute of Science

 

Collections: Biology and Medicine