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Vol. 174, No. 4JOURNAL OF BACTERIOLOGY, Feb. 1992, p. 1428-1431 0021-9193/92/041428-04$02.00/0
 

Summary: Vol. 174, No. 4JOURNAL OF BACTERIOLOGY, Feb. 1992, p. 1428-1431
0021-9193/92/041428-04$02.00/0
Copyright 1992, American Society for Microbiology
The Central Domain of Rhizobium leguminosarum DctD Functions
Independently To Activate Transcription
EVA HUALA,t JOHN STIGTER,4 AND FREDERICK M. AUSUBEL*
Department of Genetics, Harvard Medical School, and Department ofMolecular Biology,
Massachusetts General Hospital, Boston, Massachusetts 02114
Received 8 July 1991/Accepted 13 December 1991
o54-dependent transcriptional activators such as Escherichia coli NtrC, Rhizobium meliloti NifA, and
Rhizobium leguminosarum DctD share similar central and carboxy-terminal domains but differ in the structure
and function of their amino-terminal domains. We have deleted the amino-terminal and carboxy-terminal
domains ofR. leguminosarum DctD and have demonstrated that the central domain of DctD, like that of NifA,
is transcriptionally competent.
The high degree of similarity among the central domains of
a54-dependent activators (5, 8, 15) and the demonstration
that the isolated central domain of Rhizobium meliloti NifA
is capable of activating transcription (9) suggest that the
central domain is responsible for transcriptional activation.
Rhizobium leguminosarum DctD is a o-54-dependent activa-

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine