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Proc. Nati. Acad. Sci. USA Vol. 84, pp. 8558-8562, December 1987
 

Summary: Proc. Nati. Acad. Sci. USA
Vol. 84, pp. 8558-8562, December 1987
Genetics
Rhizobium meliloti has three functional copies of the nodD symbiotic
regulatory gene
(nodulation gene/plant-bacterial interaction/host specificity)
MARY A. HONMA AND FREDERICK M. AUSUBEL*
Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114
Communicated by A. M. Pappenheimer, Jr., August 13, 1987 (receivedfor review July 9, 1987)
ABSTRACT We have identified two Rhizobium meliloh
genes (nodD2 and nodD3) that are highly homologous and
closely linked to the regulatory gene nodD (nod!)). R. meliloti
strains containing mutations in the three nodD genes in all
possible combinations were constructed and their nodulation
phenotypes were assayed on Medicago sativa (alfalfa) and
Melilotus alba (sweet clover). A triple nodD1-nodD2-nodL3
mutant exhibited a Nod phenotype on alfalfaand sweet clover,
indicating that nodD is an essential nodulation gene in R.
meliloti. A nod!2 mutant exhibited no discernable defect in
nodulation and nod!3 mutants exhibited a delayed nodulation

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine