| | |
Summary: methods
A rapid, microplate SNP genotype assay for the
leptinob
allele
Angie T. Oler and Alan D. Attie1
Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706
Abstract A nonsense mutation in the mouse leptin gene
causes genetic obesity. As a result of extensive research in
the field of obesity, the use of leptinob
mice is widespread.
This mutation renders mice sterile, creating the need to
breed heterozygous mice. For this reason, leptinob
geno-
typing is necessary. To date, gel-based assays have been used
for genotyping. Using the Invader Plus: assay for single
nucleotide polymorphism (SNP) detection, we have devel-
oped a gel-free microplate SNP assay for genotyping leptinwt
and leptinob
alleles.--Oler, A. T. and A. D. Attie. A rapid,
microplate SNP genotype assay for the leptinob
|
