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Leishmania Antigens Are Presented to CD8 T Cells by a Transporter Associated with Antigen Processing-Independent
 

Summary: Leishmania Antigens Are Presented to CD8 T Cells by a
Transporter Associated with Antigen Processing-Independent
Pathway In Vitro and In Vivo
Sylvie Bertholet,1
* Romina Goldszmid,* Alexandre Morrot,* Alain Debrabant,
Farhat Afrin,2
*
Carmen Collazo-Custodio,3
* Mathieu Houde,
Michel Desjardins,
Alan Sher,* and
David Sacks4
*
CD8 T cells are generated in response to Leishmania major (Lm) or Toxoplasma gondii parasitic infections, indicating that
exogenously delivered Ag can be processed for presentation by MHC class I molecules. We show that presentation of Lm nucle-
otidase (NT)-OVA is TAP independent in vivo and in vitro, and is inhibited by chloroquine, but not by proteasome inhibitors. In
contrast, the presentation of T. gondii P30-OVA relies on the TAP/proteasome pathway. Presentation of OVA- or rNT-OVA-
coated beads also bypassed TAP requirement above a certain Ag threshold. TAP was also dispensable for the presentation of
wild-type Lm Ags to primed CD8 T cells in vitro. Finally, in vivo priming of CD8 T cells involved in acquired resistance to Lm
was not compromised in TAP-deficient mice. Thus, Leishmania Ags appear to be confined to an intraphagosomal processing

  

Source: Arnold, Jonathan - Nanoscale Science and Engineering Center & Department of Genetics, University of Georgia

 

Collections: Biotechnology