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Targeted gene expression in transgenic Xenopus using the binary Gal4-UAS system

Summary: Targeted gene expression in transgenic Xenopus
using the binary Gal4-UAS system
Katharine O. Hartley*
, Stephen L. Nutt*
, and Enrique Amaya*
*Wellcome CRC Institute, Tennis Court Road, Cambridge CB2 1QR, United Kingdom; and Department of Zoology,
University of Cambridge, Cambridge CB2 3EJ, United Kingdom
Communicated by John C. Gerhart, University of California, Berkeley, CA, December 4, 2001 (received for review October 18, 2001)
The transgenic technique in Xenopus allows one to misexpress
genes in a temporally and spatially controlled manner. However,
this system suffers from two experimental limitations. First, the
restriction enzyme-mediated integration procedure relies on chro-
mosomal damage, resulting in a percentage of embryos failing to
develop normally. Second, every transgenic embryo has unique
sites of integration and unique transgene copy number, resulting
in variable transgene expression levels and variable phenotypes.
For these reasons, we have adapted the Gal4-UAS method for
targeted gene expression to Xenopus. This technique relies on the
generation of transgenic lines that carry ``activator'' or ``effector''
constructs. Activator lines express the yeast transcription factor,


Source: Amaya, Enrique - Healing Foundation Centre & Developmental Biology, Faculty of Life Sciences, University of Manchester


Collections: Biology and Medicine