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Supporting information Puthiyaveetil et al. 10.1073/pnas.0803928105
 

Summary: Supporting information
Puthiyaveetil et al. 10.1073/pnas.0803928105
SI Text
Genotyping of SALK T-DNA Lines. The phenotypes identified and
described for chloroplast sensor kinase (CSK) are based on two
completely characterized T-DNA lines, SALK 027360 and
SALK 018074, which harbor insertions in the At1g67840 locus
encoding the CSK protein. Seeds for SALK 027360 and
SALK 018074 lines were obtained from the European Arabi-
dopsis Stock Center (Nottingham), sown on soil and the F1 plants
were selfed. A PCR based approach was used to identify
homozygous insertion lines among the F1 plants. Seeds obtained
from the genotyped, F1 homozygous plants were used to char-
acterize the phenotype. The PCR based genotyping approach
involved a genomic PCR using genomic primers, a second PCR
using genomic and T-DNA left border primers and a third
reverse transcriptase PCR (RT-PCR) to confirm the absence of
transcripts from the At1g67840 locus. The positions of the
T-DNA insertion in SALK 027360 and SALK 018074 lines are
indicated in supporting information (SI) Fig. S1. Fig. S1B shows

  

Source: Allen, John F. - School of Biological and Chemical Sciences, Queen Mary, University of London

 

Collections: Renewable Energy; Biology and Medicine