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Summary: Mot1-mediated control of transcription complex
assembly and activity
Arindam Dasgupta, Sarah A Juedes,
Rebekka O Sprouse and David T Auble*
Department of Biochemistry and Molecular Genetics, University of
Virginia Health System, Charlottesville, VA, USA
Mot1 is an essential Snf2/Swi2-related ATPase and TATA-
binding protein (TBP)-associated factor (TAF). In vitro,
Mot1 utilizes ATP hydrolysis to disrupt TBPDNA com-
plexes, but the relationship of this activity to Mot1's in
vivo function is unclear. Chromatin immunoprecipitation
was used to determine how Mot1 affects the assembly of
preinitiation complexes (PICs) at Mot1-controlled promo-
ters in vivo. We find that the Mot1-repressed HSP26 and
INO1 promoters are both regulated by TBP recruitment;
inactivation of Mot1 leads to increased PIC formation
coincident with derepression of transcription. For the
Mot1-activated genes BNA1 and URA1, inactivation of
Mot1 also leads, remarkably, to increased TBP binding to
the promoters, despite the fact that transcription of these
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