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-Adrenoceptor Agonists Stimulate Endothelial Nitric Oxide Synthase in Rat Urinary Bladder Urothelial Cells
 

Summary: -Adrenoceptor Agonists Stimulate Endothelial Nitric Oxide
Synthase in Rat Urinary Bladder Urothelial Cells
Lori A. Birder,1,2 Michele L. Nealen,4,5 Susanna Kiss,1 William C. de Groat,2 Michael J. Caterina,4,5
Edward Wang,1,3 Gerard Apodaca,1,3 and Anthony J. Kanai1,2
Laboratory of Epithelial Cell Biology, Renal-Electrolyte Division, Departments of 1Medicine, 2Pharmacology, and 3Cell
Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, and Departments of 4Biological
Chemistry and 5Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
We have investigated the intracellular signaling mechanisms un-
derlying the release of nitric oxide (NO) evoked by -adrenoceptor
(AR) agonists in urinary bladder strips and cultured bladder urothe-
lial cells from adult rats. Reverse transcription-PCR revealed that
inducible NO synthase and endothelial NOS but not neuronal NOS
genes were expressed in urothelial cells. NO release from both
urothelial cells and bladder strips was decreased (3742%) in the
absence of extracellular Ca2
(100 M EGTA) and was ablated
after incubation with BAPTA-AM (5 M) or caffeine (10 mM), indi-
cating that the NO production is mediated in part by intracellular
calcium stores. NO release was reduced (1824%) by nifedipine
(10 M) and potentiated (2932%) by incubation with the Ca2

  

Source: Apodaca, Gerard - Departments of Medicine & Cell Biology and Physiology, University of Pittsburgh

 

Collections: Biology and Medicine