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Novel gene expression domains reveal early patterning of the Xenopus endoderm

Summary: Novel gene expression domains reveal early patterning
of the Xenopus endoderm
Ricardo M.B. Costaa
, Julia Masona
, Monica Leeb
, Enrique Amayaa
, Aaron M. Zorna,b,*
Wellcome Trust/Cancer Research UK Institute of Cancer and Developmental Biology, Tennis Court Road, Cambridge, CB2 1QR, UK
Cincinnati Children's Hospital Medical Center Research Foundation. Division of Developmental Biology, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
Received 1 January 2003; received in revised form 2 February 2003; accepted 3 March 2003
The endoderm gives rise the respiratory and digestive tract epithelia as well as associated organs such as the liver, lungs and pancreas.
Investigations examining the molecular basis of embryonic endodermal patterning and organogenesis have been hampered by the lack of
regionally expressed molecular markers in the early endoderm. By differentially screening an arrayed cDNA library, combined with an in situ
hybridization screen we identified 13 new genes regionally expressed in the early tailbud endoderm of the Xenopus embryo. The putative
proteins encoded by these cDNAs include a cell surface transporter, secreted proteins, a protease, a protease inhibitor, an RNA-binding protein,
a phosphatase inhibitor and several enzymes. We find that the expression of these genes falls into one of three re-occurring domains in the
tailbud embryo; (1)a ventral midgut, (2) posterior tothe midgutand (3)in the dorsal endoderm beneath the notochord. Several of these genesare


Source: Amaya, Enrique - Healing Foundation Centre & Developmental Biology, Faculty of Life Sciences, University of Manchester


Collections: Biology and Medicine