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Magnetic Resonance Imaging Permits In Vivo Monitoring of Catheter-Based Vascular Gene Delivery
 

Summary: Magnetic Resonance Imaging Permits In Vivo Monitoring of
Catheter-Based Vascular Gene Delivery
Xiaoming Yang, MD, PhD; Ergin Atalar, PhD; Dechun Li, MD, PhD; Jean-Michel Serfaty, MD;
Danming Wang, MD; Ananda Kumar, MS; Linzhao Cheng, PhD
Background--Gene therapy is an exciting frontier in modern medicine. To date, most investigations about the imaging of
gene therapy have primarily focused on noncardiovascular systems, and no in vivo imaging modalities are currently
available for monitoring vascular gene therapy. The purpose of this study was to develop an in vivo imaging tool to
monitor a catheter-based vascular gene delivery procedure.
Methods and Results--We produced gadolinium/blue dye and gadolinium/gene-vector media by mixing Magnevist with
a trypan-blue or a lentiviral vector carrying a green fluorescent protein (GFP) gene. The gadolinium was used as an
imaging marker for magnetic resonance (MR) imaging to visualize vessel wall enhancement, and the blue dye/GFP was
used as a tissue stain marker for histology/immunohistochemistry to confirm the success of the transfer. Using Remedy
gene delivery catheters, we transferred the gadolinium/blue dye (n 8) or gadolinium/GFP lentivirus (n 4) into the
arteries of 12 pigs, that were monitored under high-resolution MR imaging. The results showed, in all 12 pigs, the
gadolinium enhancement of the target vessel walls on MR imaging and the blue/GFP staining of the target vessel tissues
with histology/immunohistochemistry. This study shows the potential of using MR imaging to dynamically visualize (1)
where the gadolinium/genes are delivered; (2) how the target portion is marked; and (3) whether the gene transfer
procedure causes complications.
Conclusions--We present a technical development that uses high-resolution MR imaging as an in vivo imaging tool to
monitor catheter-based vascular gene delivery. (Circulation. 2001;104:1588-1590.)

  

Source: Atalar, Ergin - Department of Electrical and Electronics Engineering, Bilkent University

 

Collections: Engineering; Biology and Medicine