Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
TATA-binding Protein Variants That Bypass the Requirement for Mot1 in Vivo*S
 

Summary: TATA-binding Protein Variants That Bypass the Requirement
for Mot1 in Vivo*S
Received for publication,November 25, 2008, and in revised form, December 18, 2008 Published, JBC Papers in Press,December 21, 2008, DOI 10.1074/jbc.M808951200
Rebekka O. Sprouse, Melissa N. Wells1
, and David T. Auble2
From the Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908
Mot1 is an essential TATA-binding protein (TBP)-associated
factor and Snf2/Swi2 ATPase that both represses and activates
transcription. Biochemical and structural results support a
model in which ATP binding and hydrolysis induce a conforma-
tional change in Mot1 that drives local translocation along
DNA, thus removing TBP. Although this activity explains tran-
scriptional repression, it does not as easily explain Mot1-medi-
ated transcriptional activation, and several different models
have been proposed to explain how Mot1 activates transcrip-
tion. To better understand the function of Mot1 in yeast cells in
vivo, particularly with regard to gene activation, TBP mutants
were identified that bypass the requirement for Mot1 in vivo.
Although TBP has been extensively mutated and analyzed pre-
viously, this screen uncovered two novel TBP variants that are

  

Source: Auble, David - Department of Biochemistry and Molecular Genetics, University of Virginia

 

Collections: Biology and Medicine