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letters to nature NATURE |VOL 410 |5 APRIL 2001 |www.nature.com 701

Summary: letters to nature
NATURE |VOL 410 |5 APRIL 2001 |www.nature.com 701
roughly 300 animals, and given a choice between an optimal concentration of benzalde-
hyde (1:200 dilution in ethanol) and a lower concentration of diacetyl (1:10,000 dilution in
ethanol) in the presence of a uniform Želd of butanone (1.2 ml per 10-ml plate). Under
these conditions more than 95% of wild-type animals prefer benzaldehyde. Animals that
accumulated at the diacetyl source were removed and retested under the same conditions
to repeat the enrichment. Animals that preferred diacetyl three times were isolated, and
their F3 broods were given a choice between benzaldehyde and diacetyl in the absence of a
uniform concentration of butanone. Mutants that could chemotax to benzaldehyde under
these conditions were saved. Twenty-seven mutants exhibited discrimination defects that
could also be replicated without the diacetyl counterattractant. Mutants were backcrossed
twice to wild-type animals.
Genetic mapping of ky542
We mapped ky542 to chromosome II by observing segregation of the discrimination
phenotype away from the dominant marker sqt-1(sc1) (7/7 isolates). Subsequent mapping
was performed by following segregation of the discrimination phenotype with single-
nucleotide polymorphisms (SNPs) between the wild-type N2 and CB4856 strains. F2
progeny of ky542 ´ CB4856 crosses were isolated, and populations were generated from
each isolate. Each population was tested for butanone/benzaldehyde discrimination.


Source: Akabas, Myles - Department of Physiology and Biophysics, Albert Einstein College of Medicine, Yeshiva University


Collections: Biology and Medicine