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Phytologia (April 2010) 92(1)82 SCREENING FOR LOW-COPY NUCLEAR GENE REGIONS IN
 

Summary: Phytologia (April 2010) 92(1)82
SCREENING FOR LOW-COPY NUCLEAR GENE REGIONS IN
THE CUPRESSACEAE
Robert P. Adams
Biology Department, Baylor University, Waco, TX 76798
Robert_Adams@baylor.edu
ABSTRACT
Thirty two low-copy nuclear (LCN) gene regions from Steele
et al. (2008) were amplified using Hesperocyparis goveniana
(Cupressaceae) DNA. Ten of these gave successfully amplified
products and these were sequenced. Four of these LCNs yielded clean
sequences. Of the 4 LCNs, one proved to be a mitochondrion gene
region with no variation between Hesperocyparis and Juniperus;. The
second LCN, tentatively identified as Actin-11, gave 497 bp of clean
sequence then appeared to be multi-copy. The third LCN, tentatively
identified as part of 3-carene synthase, gave 924 bp of clean sequence
in Hesperocyparis but appeared to be multi-copy in Juniperus. The
fourth LCN, possibly heat shock protein 90.1, yielded 946 bp of good
sequence data for H. goveniana, J. scopulorum and J. virginiana and
appears to offer promise for utilization in Cupressaceae taxonomy.

  

Source: Adams, Robert P. - Department of Biology, Baylor University

 

Collections: Biology and Medicine