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Summary: Tuesday, September 02, 2008
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Replication, Variation and Normalization in Microarray Experiments
Naomi Altman
Department of Statistics
Pennsylvania State University
State College, PA 16802-2111
Abstract
Issues in the design and analysis of microarray experiments can be illuminated by
statistical modeling. In this paper, a linear mixed model is used to determine the
implications of replication of various types including technical and biological replication,
dye-swapping and sample pooling, and of analysis tools such as centering and
normalization. The discussion applies to both 2-color and 1-color systems such as
Affymetrix@ arrays.
Key words: centering; sample pooling; mixed model; experiment design, microarray
design, microarray analysis
contact information: nsa1@psu.edu; fax: 814-863-7114
running head: Microarray Replication, Variation, Normalization
Tuesday, September 02, 2008
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