Summary: © The Rockefeller University Press, 0021-9525/97/04/5/14 $2.00
The Journal of Cell Biology, Volume 137, Number 1, April 7, 1997 5­18 5
Telomeres Cluster De Novo before the Initiation of Synapsis:
A Three-dimensional Spatial Analysis of Telomere Positions
before and during Meiotic Prophase
Hank W. Bass,* Wallace F. Marshall,
John W. Sedat,
David A. Agard,§
and W. Zacheus Cande*
*Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720; and
Department
of Biochemistry and Biophysics, §
Howard Hughes Medical Institute, University of California at San Francisco, San Francisco,
California 94143
Abstract. We have analyzed the progressive changes in
the spatial distribution of telomeres during meiosis us-
ing three-dimensional, high resolution fluorescence mi-
croscopy. Fixed meiotic cells of maize (Zea mays L.)
were subjected to in situ hybridization under conditions
that preserved chromosome structure, allowing identifi-

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco
Bass, Hank W. - Department of Biological Science, Florida State University

Collections: Biology and Medicine; Biotechnology