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Development of immortal introgression populations; genome wide Near Isogenic Lines (NILs) for cotton

Summary: Development of immortal introgression populations; genome wide Near Isogenic Lines
(NILs) for cotton
Rahul Chandnani
Crop and Soil Sciences Seminar
Wednesday March 9, 2011 at 3:35 pm
Room 2401, Miller Plant Sciences Building
Cotton is the world's foremost textile fiber crop and contributes to a multibillion textile
industry. Cotton fiber has been the focal point of most research efforts in cotton. The mystery
of fiber development by the elongation of a single cell remains unsolved. Therefore, various
breeding efforts have been conducted for a long time to develop mapping populations so that
dissection of gene networks responsible for the functional genomics behind fiber development
can be accomplished. The objective of our research is to develop immortal introgression
mapping populations; genome wide Near Isogenic Lines (NILs) for cotton. NILs enable the
mapping of QTL with small phenotypic effect and also, reduced background noise. Further
NILs provide the platform to study the effect of epistatic interaction on the phenotype. We are
aiming to develop two reciprocal set of NILs by introgression between Acala Maxxa
(Gossypium hirsutum) and Pima S6 (Gossypium barbadenes). The ultimate population that will
be used for genotyping is BC4. A total of 192 BC4 plants in each set of NILs will contain a
single introgressed segment of the donor genome but will collectively `tile' the genome. A
population with a sufficient number of backcross lineages is expected to provide 3x coverage of


Source: Arnold, Jonathan - Nanoscale Science and Engineering Center & Department of Genetics, University of Georgia


Collections: Biotechnology