Carbohydrate SAMs Characterized by Sum-Frequency Generation
Achani Yatawara, Gopinath Tiruchinapally, Andrey N. Bordenyuk, Peter R. Andreana
and Alexander V. Benderskii*
Department of Chemistry, Wayne State University, Detroit, MI 48202
Materials and Methods: All reagents were purchased from Aldrich unless otherwise
mentioned. HOBT and HBTU were purchased from Alfa Aesar. Except as otherwise
indicated, reactions were carried out under argon atmosphere. All reactions were
monitored using thin layer chromatography on 0.25 mm Dynamic Adsorbents, L.L.C.
precoated silica gel (particle size 0.03-0.07 mm, catalog no. 84111, lot #LA2006).
Column chromatography was performed using Whatman Purasil 60 (230-400 mesh
ASTM) silica gel. Yields refer to chromatographically and spectroscopically pure
compounds except where noted. A Thermolyne, Maxi-Mix III type 65800 was used for
shaking gold slides with the appropriate linker. BD Falcon Petri dishes (50 x 9 mm) from
BD Biosciences purchased through VWR was used to house the gold slides and media for
H and 13
C NMR spectra were recorded on Varian Unity 300, Varian Mercury