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Engineering proteins that bind, move, make and break DNA Cynthia H Collins

Summary: Engineering proteins that bind, move, make and break DNA
Cynthia H Collins√
, Yohei Yokobayashiy
, Daisuke Umenoy
Frances H Arnoldyz
Recent protein engineering efforts have generated artificial
transcription factors that bind new target DNA sequences and
enzymes that modify DNA at new target sites. Zinc-finger-based
transcription factors are favored targets for design; important
technological advances in their construction and numerous
biotechnological applications have been reported. Other notable
advances include the generation of endonucleases and
recombinases with altered specificities, made by innovative
combinatorial and evolutionary protein engineering strategies.
An unexpectedly high tolerance to mutation in the active sites of
DNA polymerases is being exploited to engineer polymerases to
incorporate artificial nucleotides or to display other, nonnatural


Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology


Collections: Chemistry; Biology and Medicine