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Differential Presynaptic Localization of Metabotropic Glutamate Receptor Subtypes in the Rat Hippocampus
 

Summary: Differential Presynaptic Localization of Metabotropic Glutamate
Receptor Subtypes in the Rat Hippocampus
Ryuichi Shigemoto,1 Ayae Kinoshita,1 Eiki Wada,1 Sakashi Nomura,3 Hitoshi Ohishi,1 Masahiko Takada,1
Peter J. Flor,4 Akio Neki,2 Takaaki Abe,2 Shigetada Nakanishi,2 and Noboru Mizuno1
Departments of 1Morphological Brain Science and 2Biological Sciences, Faculty of Medicine, and 3College of Medical
Technology, Kyoto University, Kyoto 606, Japan, and 4Novartis Pharma Inc., Nervous System Research,
CH-4002 Basel, Switzerland
Neurotransmission in the hippocampus is modulated variously
through presynaptic metabotropic glutamate receptors
(mGluRs). To establish the precise localization of presynaptic
mGluRs in the rat hippocampus, we used subtype-specific
antibodies for eight mGluRs (mGluR1­mGluR8) for immunohis-
tochemistry combined with lesioning of the three major hip-
pocampal pathways: the perforant path, mossy fiber, and
Schaffer collateral. Immunoreactivity for group II (mGluR2) and
group III (mGluR4a, mGluR7a, mGluR7b, and mGluR8) mGluRs
was predominantly localized to presynaptic elements, whereas
that for group I mGluRs (mGluR1 and mGluR5) was localized to
postsynaptic elements. The medial perforant path was strongly
immunoreactive for mGluR2 and mGluR7a throughout the hip-

  

Source: Alford, Simon - Department of Biological Sciences, University of Illinois at Chicago

 

Collections: Biology and Medicine