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The RACK1 signal anchor protein from Trypanosoma brucei associates with eukaryotic elongation factor 1A: a role for
 

Summary: The RACK1 signal anchor protein from Trypanosoma brucei
associates with eukaryotic elongation factor 1A: a role for
translational control in cytokinesis
Sandesh Regmi, Karen G. Rothberg,
James G. Hubbard and Larry Ruben*
Department of Biological Sciences, Southern Methodist
University, Dallas, TX 75275, USA.
Summary
RACK1 is a WD-repeat protein that forms signal com-
plexes at appropriate locations in the cell. RACK1
homologues are core components of ribosomes from
yeast, plants and mammals. In contrast, a cryo-EM
analysis of trypanosome ribosomes failed to detect
RACK1, thus eliminating an important translational
regulatory mechanism. Here we report that TbRACK1
from Trypanosoma brucei associates with eukaryotic
translation elongation factor-1a (eEF1A) as deter-
mined by tandem MS of TAP-TbRACK1 affinity
eluates, co-sedimentation in a sucrose gradient, and
co-precipitation assays. Consistent with these obser-

  

Source: Aksoy, Serap - School of Public Health, Yale University

 

Collections: Biology and Medicine