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Temporaland Spatial Coordination of Chromosome Movement, Spindle Formation, and Nuclear Envelope Breakdown
 

Summary: Temporaland Spatial Coordination of Chromosome Movement,
Spindle Formation, and Nuclear Envelope Breakdown
During Prometaphase in DrosophilamelanogasterEmbryos
Yasushi Hiraoka, David A. Agard, and John W. Sedat
Department ofBiochemistry and Biophysicsand the HowardHughes Medical Institute,
University ofCalifornia, San Francisco, San Francisco, California 94143-0554
Abstract. The spatial and temporal dynamics of
diploid chromosome organization, microtubule ar-
rangement, and the state of the nuclear envelope have
been analyzed in syncytial blastoderm embryos of
Drosophila melanogaster during the transition from
prophase to metaphase, by three-dimensional optical
sectioning microscopy. Time-lapse, three-dimensional
data recorded in living embryos revealed that congres-
sion of chromosomes (the process whereby chromo-
somes move to form the metaphase plate) at
prometaphase occurs as a wave, starting at the top of
the nucleus near the embryo surface and proceeding
through the nucleus to the bottom. The time-lapse
analysis was augmented by a high-resolution analysis

  

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

 

Collections: Biotechnology; Biology and Medicine