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Boric acid reversibly inhibits the second step of pre-mRNA splicing Noam Shomron, Gil Ast
 

Summary: Boric acid reversibly inhibits the second step of pre-mRNA splicing
Noam Shomron, Gil Ast√
Department of Human Genetics and Molecular Medicine, Sackler School of Medicine, Tel Aviv University, Ramat Aviv 69978, Tel Aviv, Israel
Received 14 July 2003; revised 15 August 2003; accepted 15 August 2003
First published online 27 August 2003
Edited by Felix Wieland
Abstract Several approaches have been used to identify the
factors involved in mRNA splicing. None of them, however,
comprises a straightforward reversible method for inhibiting
the second step of splicing using an external reagent other
than a chelator. This investigation demonstrates that the addi-
tion of boric acid to an in vitro pre-mRNA splicing reaction
causes a dose-dependent reversible inhibition e°ect on the sec-
ond step of splicing. The mechanism of action does not involve
chelation of several metal ions; hindrance of 3PP splice-site; or
binding to hSlu7. This study presents a novel method for speciĘc
reversible inhibition of the second step of pre-mRNA splicing.
Ŗ 2003 Published by Elsevier B.V. on behalf of the Federation
of European Biochemical Societies.
Key words: Pre-mRNA splicing; Inhibition; Boric acid;

  

Source: Ast, Gil - Department of Molecular Genetics and Biochemistry, Tel Aviv University

 

Collections: Biology and Medicine