Use of dimethylsulfate to probe RNA structure in vivo
Sandra E. Wells, John M. X. Hughes, A. Haller Igel and Manuel Ares, Jr.
Center for the Molecular Biology of RNA
Department of Biology
University of California, Santa Cruz
Santa Cruz, CA 95064
Telephone 831 459 4917
FAX 831 459 3737
Published in Methods in Enzymology Vol. 318, pp 479-493 (2000)
Understanding how RNA works requires the coordinated use of diverse experimental
approaches. Knowledge of RNA structure and its relationship to function is an essential ingredient
for interpreting the biological mechanisms of RNA action. Use of genetic, phylogenetic,
biophysical, and computational approaches to divining RNA structure and structural dynamics is
greatly enhanced by the application of chemical and enzymatic probes of RNA structure in solution.
Among the most versatile chemical probes available for studying RNA and ribonucleoprotein