| | |
Summary: ation for FISH as 60% (0.75 0.95 0.85). The
validity of this estimation was demonstrated when
the same calculation was applied to GFP experi-
ments. The maximum detection of acentric minichro-
matid dissociation was estimated as 39%, a value
very similar to the actual measured value.
11. P. Megee and D. Koshland, unpublished observation.
12. J. H. Hegemann and U. N. Fleig, Bioessays 15, 451
(1993).
13. A. A. Hyman and P. K. Sorger, Annu. Rev. Cell Dev.
Biol. 11, 471 (1995).
14. M. Saunders, M. Fitzgerald-Hayes, K. Bloom, Proc.
Natl. Acad. Sci. U.S.A. 85, 175 (1988).
15. P. B. Meluh and D. Koshland, Genes Dev. 11, 3401
(1997).
16. P. K. Sorger et al., Proc. Natl. Acad. Sci. U.S.A. 92,
12026 (1995).
17. Supported in part by NIH grant GM41718. We thank
M. K. Raghuraman and R. Ciosk for plasmids and C.-M.
Fan, V. Guacci, P. Meluh, and members of the Ko-
|
