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APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Sept. 2002, p. 44254430 Vol. 68, No. 9 0099-2240/02/$04.00 0 DOI: 10.1128/AEM.68.9.44254430.2002
 

Summary: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Sept. 2002, p. 44254430 Vol. 68, No. 9
0099-2240/02/$04.00 0 DOI: 10.1128/AEM.68.9.44254430.2002
Copyright 2002, American Society for Microbiology. All Rights Reserved.
Environmental Factors That Control Microbial Perchlorate Reduction
Swades K. Chaudhuri, Susan M. O'Connor, Ruth L. Gustavson, Laurie A. Achenbach, and
John D. Coates*
Department of Microbiology, Southern Illinois University, Carbondale, Illinois 62901
Received 11 April 2002/Accepted 27 June 2002
As part of a study to elucidate the environmental parameters that control microbial perchlorate respiration,
we investigated the reduction of perchlorate by the dissimilatory perchlorate reducer Dechlorosoma suillum
under a diverse set of environmental conditions. Our results demonstrated that perchlorate reduction by D.
suillum only occurred under anaerobic conditions in the presence of perchlorate and was dependent on the
presence of molybdenum. Perchlorate reduction was dependent on the presence of the enzyme chlorite
dismutase, which was induced during metabolism of perchlorate. Anaerobic conditions alone were not enough
to induce expression of this enzyme. Dissolved oxygen concentrations less than 2 mg liter 1
were enough to
inhibit perchlorate reduction by D. suillum. Similarly to oxygen, nitrate also regulated chlorite dismutase
expression and repressed perchlorate reduction by D. suillum. Perchlorate-grown cultures of D. suillum
preferentially reduced nitrate in media with equimolar amounts of perchlorate and nitrate. In contrast, an
extended (40 h) lag phase was observed if a similar nitrate-perchlorate medium was inoculated with a

  

Source: Achenbach, Laurie A. - Department of Microbiology, Southern Illinois University

 

Collections: Environmental Sciences and Ecology; Environmental Management and Restoration Technologies; Biology and Medicine