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Structure-guided SCHEMA recombination of distantly related b-lactamases
 

Summary: Structure-guided SCHEMA recombination of
distantly related b-lactamases
Michelle M. Meyer1
, Lisa Hochrein2
and
Frances H. Arnold12,3
1
Biochemistry and MolecularBiophysics,CaliforniaInstituteof Technology,
Mail Code 210-21, and 2
Division of Chemistry and Chemical Engineering,
California Institute of Technology, Mail Code 210-41, Pasadena, CA
91125, USA
3
To whom correspondence should be addressed.
E-mail: frances@cheme.caltech.edu
We constructed a library of b-lactamases by recombining
three naturally occurring homologs (TEM-1, PSE-4,
SED-1) that share 3442% sequence identity. Most
chimeras created by recombining such distantly related
proteins are unfolded due to unfavorable side-chain inter-

  

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

 

Collections: Chemistry; Biology and Medicine