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-Subunit of cardiac Na -K -ATPase dictates the concentration of the functional enzyme in caveolae
 

Summary: -Subunit of cardiac Na -K -ATPase dictates the concentration of the
functional enzyme in caveolae
Lijun Liu and Amir Askari
Department of Physiology, Pharmacology, Metabolism, and
Cardiovascular Sciences, Medical University of Ohio, Toledo, Ohio
Submitted 4 January 2006; accepted in final form 16 April 2006
Liu, Lijun, and Amir Askari. -Subunit of cardiac Na -K -
ATPase dictates the concentration of the functional enzyme in caveo-
lae. Am J Physiol Cell Physiol 291: C569C578, 2006. First pub-
lished April 19, 2006; doi:10.1152/ajpcell.00002.2006.--Previous
studies showed the presence of a significant fraction of Na -K -
ATPase -subunits in cardiac myocyte caveolae, suggesting the
caveolar interactions of Na -K -ATPase with its signaling partners.
Because both - and -subunits are required for ATPase activity, to
clarify the status of the pumping function of caveolar Na -K -
ATPase, we have examined the relative distribution of two major
subunit isoforms ( 1 and 1) in caveolar and noncaveolar membranes
of adult rat cardiac myocytes. When cell lysates treated with high salt
(Na2CO3 or KCl) concentrations were fractionated by a standard
density gradient procedure, the resulting light caveolar membranes

  

Source: Askari, Amir - Department of Biochemistry & Cancer Biology, University of Toledo
Askari, Amir - Department of Physiology and Pharmacology, University of Toledo

 

Collections: Biology and Medicine