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Brief Communication 1195 A gene trap approach in Xenopus

Summary: Brief Communication 1195
A gene trap approach in Xenopus
Odile J. Bronchain, Katharine O. Hartley and Enrique Amaya
The frog transgenesis technique ultimately promises to
make mutagenesis possible through random insertion
of plasmid DNA into the genome. This study was
undertaken to evaluate whether a gene trap approach
combined with transgenesis would be appropriate for
performing insertional mutagenesis in Xenopus
embryos. Firstly, we confirmed that the transgenic
technique results in stable integration into the genome
and that transmission through the germline occurs in
the expected Mendelian fashion. Secondly, we
developed several gene trap vectors, using the green
fluorescent protein (GFP) as a marker. Using these
vectors, we trapped several genes in Xenopus laevis
that are expressed in a spatially restricted manner,
including expression in the epiphysis, the olfactory bulb
and placodes, the eyes, ear, brain, muscles, tail and
intestine. Finally, we cloned one of the trapped genes


Source: Amaya, Enrique - Healing Foundation Centre & Developmental Biology, Faculty of Life Sciences, University of Manchester


Collections: Biology and Medicine