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Yeast Idiosyncrasies From Cora Styles (Fink Lab)

Summary: 1
Yeast Idiosyncrasies
From Cora Styles (Fink Lab)
ade1 and ade2 cultures turn red. The intensity depends on the amount of adenine in the medium.
YPD is not rich in adenine, so cultures turn red in 2-3 days. Petites don't turn red.
asp5 requires asparagine added to YPD ascospore dissection plate. Extra asp needed only for
germination; cultures grow normally on YPD. asp5 turns red in aged cultures; not as fast or
intense as ade2 or ade1.
gcn4 has an arginine requirement (partial?) canr (arginine permease) makes gcn4 grow very slowly.
trp slows growth, especially at lower temperatures. Extra trp in medium helps, but does not restore
wildtype growth. Different mutants may show a range of phenotypes.
Petites may be rho minus or rho zero. Colonies are smaller and white, and their size can vary over
a wide range in one culture.
Dissections Usually done on YPD. Some slow-growing mutants (sit, eg.) show increased
germination frequency on GNA; other mutants germinate worse (report Botstein
lab) on GNA.
Drop in The amount of amino acid stock solutions recommended for supplementing minimal
medium: YNB (SD in the literature) is based on empirical and practical factors, and allows
mutants with a single auxotrophy to grow like wild-type. Minimal medium with one to
several additions is called drop-in.


Source: Aris, John P. - Department of Anatomy and Cell Biology, University of Florida


Collections: Biology and Medicine