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Postnatal Maturation of Gephyrin/ Glycine Receptor Clusters on Developing
 

Summary: Postnatal Maturation of Gephyrin/
Glycine Receptor Clusters on Developing
Renshaw Cells
ERIC J. GEIMAN, MICHAEL C. KNOX, AND FRANCISCO J. ALVAREZ*
Department of Anatomy, Wright State University, Dayton, Ohio 45435
ABSTRACT
Adult mammalian Renshaw cells express large and complex postsynaptic gephyrin/
glycine receptor clusters on their surface. Larger gephyrin clusters correlate with more
"efficacious" inhibitory synapses, in terms of larger postsynaptic quantal size amplitudes, in
part because they likely contain more postsynaptic receptors (Lim et al. [1999] J. Physiol.
(Lond.) 516:505512; Oleskevich et al. [1999] J. Neurophysiology 82:312319). Here, we
studied the postnatal development of the gephyrin/glycine receptor cluster size on Renshaw
cells. Renshaw cells were identified by their calbindin immunoreactivity, location and mor-
phology, and presence of cholinergic input. The populations of clusters over developing
Renshaw cells immunoreactive to gephyrin or glycine receptor 1 subunits were comparable
in number, size, and complexity and displayed a high degree of colocalization ( 90%) at all
ages. Quantitative morphologic analysis was performed on gephyrin-immunoreactive clusters. In
neonatal animals, Renshaw cells expressed small punctate gephyrin-immunoreactive clusters
(mean cluster size SD 0.19 0.19 m2
at 2 days; 0.22 0.19 m2

  

Source: Alvarez, Francisco J. - Department of Neuroscience, Cell Biology, and Physiology, Wright State University

 

Collections: Biology and Medicine