Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
clinical trials of bone marrow progenitor cells for cardiac repair have been initiated over the last 2 yr16,17
 

Summary: clinical trials of bone marrow progenitor cells for cardiac repair
have been initiated over the last 2 yr16,17
. The failure of HSCs to
contribute significantly to formation of new cardiomyocytes in the
present study may call into question the mechanistic underpinnings
of such trials. A
Methods
Isolation of bone-marrow-derived HSCs
Tibias, femurs and iliac crests were collected from MHCnLAC, MHCEGFP or
b-ActEGFP mice, crushed in PBS containing 0.1% BSA and filtered through a 40-mm
nylon mesh to obtain crude bone marrow. Crude marrow was then fractionated on
Histopaque (1.083 g ml21
, Sigma) at 740g for 25 min to collect low-density marrow cells
from the interface. Both mature and immature haematopoietic cells were depleted from
low-density marrow cells by pre-incubation with lineage-specific rat antibodies to murine
CD4, CD8, Gr-1, B220 and Mac-1 (Pharmingen) and subsequently labelling with anti-rat
IgG microbeads followed by magnetic cell sorting (MACS, Miltenyi Biotech). Briefly,
unlabelled progenitor cells were separated from magnetically labelled low-density marrow
cells on a column, which was placed in the magnetic field of a MACS separator. The
magnetically labelled cells were retained in the column. Cells (Lin-depleted) present in the

  

Source: Akabas, Myles - Department of Physiology and Biophysics, Albert Einstein College of Medicine, Yeshiva University

 

Collections: Biology and Medicine