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Summary: ON IDENTIFYING INFORMATION FROM IMAGE-BASED SPATIAL POLARITY
PHENOTYPES IN NEUTROPHILS
Chin-Jen Ku*
, Yanqin Wang*
, Benjamin Pavie, Steven J. Altschuler, Lani F. Wu
University of Texas Southwestern Medical Center. Dallas, Texas 75390. U.S.A.
Department of Pharmacology, Green Center for Systems Biology
e-mails: {chin-jen.ku, yanqin.wang, benjamin.pavie, steven.altschuler, lani.wu}@utsouthwestern.edu
ABSTRACT
Cell polarity is involved in many biological functions such
as development, wound healing and immune responses. In
human neutrophils, polarization is characterized by the
translocation of distinct sets of signaling molecules to
opposite ends of the cell and the rapid rearrangement of
cytoskeleton to initiate migration. While many image-based
studies have described cellular morphology and the intensity
level of polarity signaling molecules, systematic
characterization of the spatial distribution of polarity
signaling molecules has been lacking. Here we designed a
collection of analytical features to quantify spatial
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