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Proc. Nati Acad. Sci. USA Vol. 80, pp. 4030-4034, July 1983
 

Summary: Proc. Nati Acad. Sci. USA
Vol. 80, pp. 4030-4034, July 1983
Genetics
Activation of Klebsiellapneumoniae and Rhizobium meliloti
nitrogenase promoters by gin (ntr) regulatory proteins
(nitrogen metabolism regulation/symbiotic nitrogen fixation/lac fusions)
VENKATESAN SUNDARESAN*t, DAVID W. OWtt, AND FREDERICK M. AUSUBELtt
*Committee on Biophysics and tDepartment of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138
Communicated by HaroldJ. Evans, March 11, 1983
ABSTRACT We have studied the expression, in different
Escherichia coli gin (ntr) mutants, of fusions (constructed in vitro)
of the nifHDK (nitrogenase) promoters from Klebsiela pneumon-
iae and Rhizobium meliloti to E. coli lacZ. Derepression of the K.
pneumoniae nifH::lacZ fusion requires the glnF (ntrA) gene prod-
uct in addition to the K. pneumoniae nifA gene product, indicating
that regulation of the K. pneumoniae'nifgenes is more closely in-
tegrated with the overall nitrogen control system than previously
demonstrated. Derepression of the R. meliloti nifH::IacZ fusion
in E. coli by the K. pneumoniae nifA gene product (which we had
previously shown) exhibits the same requirement for glnF. De-

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine