Sprouse et al. Supplemental Data
Supplementary Figure 1: Comparison of mot1-14 microarray datasets from Dasgupta et al.
(2002) (1) and the present study. The data sets are well correlated in that the vast majority of the
Mot1-regulated genes identified in the two studies are regulated by Mot1 in the same direction.
A modest correlation value is in large part attributable to improvements in technology, resulting
in quantitative differences in the effects of a MOT1 mutation.
Supplementary Figure 2: EMSA using radiolabeled ARG3 promoter DNA (< 1 nM). ARG3 is a
Mot1-repressed promoter whose expression was restored to WT levels by both TBP Y185C and
TBP F207L. The experiment was performed as in Figure 3. 10 nM of the WT or mutant TBP
proteins was used; the reaction in lane 1 contained DNA alone. The TBP-DNA complex is
Supplementary Table 1: Comparison with bur6-2 and spt20 microarray datasets
% (#) Genes