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Electrophoresis Buffers And Solutions Use ACS grade chemicals and dH2O for all buffers.
 

Summary: Electrophoresis Buffers And Solutions
NOTE:
Use ACS grade chemicals and dH2O for all buffers.
Use Sigma Trizma Tris Base and Tris-HCl for all buffers.
SDS-PAGE 5X Resolving Buffer 500 ml
Tris-HCl (Sigma) 27.5 g
Tris-Base (Sigma) 100 g
SDS (BioRad) 2.5 g
Filter through 0.45 m.
SDS-PAGE 5X Stacking buffer 500 ml
Tris-HCl (Sigma) 38.5 g
Tris-Base (Sigma) 1.5 g
SDS (BioRad) 2.5 g
Filter through 0.45 m.
30% Acrylamide Solution For SDS-PAGE: 200 ml
60 g Acrylamide
1.6 g Methylene Bis acrylamide (1:37.5)
Bring to 200 ml with dH2O
Filter through 0.45 m. Store at 4C. Expose to light as little as possible.
SDS PAGE Sample Buffer:

  

Source: Aris, John P. - Department of Anatomy and Cell Biology, University of Florida

 

Collections: Biology and Medicine