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Proc. NatL. Acad. Sci. USA Vol. 79, pp. 5577-5581, September 1982
 

Summary: Proc. NatL. Acad. Sci. USA
Vol. 79, pp. 5577-5581, September 1982
Cell Biology
Cell cycle stage-specific accumulation of mRNAs encoding tubulin
and other polypeptides in Chlamydomonas
(mitosis/cDNA cloning/blot hybridization technique/regulation of mRNA accumulation/synchronous cells)
MANUEL ARES, JR.* AND STEPHEN H. HOWELLt
Biology Department, C-016, University ofCalifornia at San Diego, La Jolla, California 92093
Communicated by Morris Friedkin, June 16, 1982
ABSTRACT The accumulation pattern ofanumberofmRNAs
during the cell cycle ofChlamydomonas was examined by two-di-
mensional gel analysis ofin vitro translation products and by RNA
blothybridization analysis. Two-dimensional gel analysis revealed
that 10-15% of the 300 most abundant translation products are
differentially synthesized from RNA obtained at various cell cycle
stages. RNAs that direct the synthesis of a- and 8-tubulins and
that hybridize to cloned a- and /8-tubulin probes accumulate co-
ordinately during the predivision period ofthe cell cycle, reaching
peak levels before or during division. Other RNAs represented
by selected cloned cDNA probes show a number of different cell

  

Source: Ares Jr., Manny - Department of Molecular, Cell, and Developmental Biology, University of California at Santa Cruz

 

Collections: Biology and Medicine