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LQG-based tracking of multiple fluorescent particles in two-dimensions in a confocal microscope
 

Summary: LQG-based tracking of multiple fluorescent particles in two-dimensions
in a confocal microscope
Zhaolong Shen and Sean B. Andersson
Abstract-- This paper describes a scheme to track multiple
fluorescent particles diffusing in two dimensions. Fluorescence
intensities are measured using a scanning-stage confocal setup.
We develop a combined model for the position of the particles
and the dynamics of the piezo-stage and design a linear-
quadratic-Gaussian controller to drive the overall estimation
error to zero. The scheme is illustrated through simulation.
I. INTRODUCTION
Particle tracking is an important class of tools for studying
single-molecule systems in molecular biology. Based on the
number of particles to track, algorithms can be divided
into two main categories: single particle tracking (SPT)
and multiple particle tracking (MPT). SPT has been used
in the study of many systems and has been shown useful
in measuring diffusion coefficients [1], in discriminating
molecules according to their mobility [2] and in revealing
dynamics of signaling receptors [3]. Most approaches rely

  

Source: Andersson, Sean B. - Department of Aerospace and Mechanical Engineering, Boston University

 

Collections: Engineering