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Summary: UCSF tomography: An integrated software suite
for real-time electron microscopic tomographic
data collection, alignment, and reconstruction
Shawn Q. Zheng a,b,1
, Bettina Keszthelyi b,1
, Eric Branlund a,b,1
, John M. Lyle b
,
Michael B. Braunfeld a,b
, John W. Sedat b
, David A. Agard a,b,*
a
The Howard Hughes Medical Institute, University of California, San Francisco, CA 94158-2517, USA
b
The W.M. Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California, San Francisco,
CA 94158-2517, USA
Received 22 March 2006; received in revised form 8 June 2006; accepted 11 June 2006
Abstract
A real-time alignment and reconstruction scheme for electron microscopic tomography (EMT) has been developed and integrated
within our UCSF tomography data collection software. This newly integrated software suite provides full automation from data collec-
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