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A high-throughput, near-saturating screen for type III effector genes from Pseudomonas syringae
 

Summary: A high-throughput, near-saturating screen for type III
effector genes from Pseudomonas syringae
Jeff H. Chang*, Jonathan M. Urbach
, Terry F. Law*, Larry W. Arnold
, An Hu§
, Saurabh Gombar*, Sarah R. Grant*¶
,
Frederick M. Ausubel
, and Jeffery L. Dangl*¶
Departments of *Biology CB#3280 and Microbiology and Immunology CB#7290, and ¶Curriculum in Genetics, Carolina Center for Genome Sciences,
University of North Carolina, Chapel Hill, NC 27599 ; Department of Molecular Biology, Massachusetts General Hospital, Cambridge, MA 02139; and
§Syngenta Biotechnology, Inc., P.O. Box 12257, 3054 Cornwallis Road, Research Triangle Park, NC 27709
Communicated by Brian J. Staskawicz, University of California, Berkeley, CA, December 22, 2004 (received for review November 4, 2004)
Pseudomonas syringae strains deliver variable numbers of type III
effector proteins into plant cells during infection. These proteins
are required for virulence, because strains incapable of delivering
them are nonpathogenic. We implemented a whole-genome, high-
throughput screen for identifying P. syringae type III effector
genes. The screen relied on FACS and an arabinose-inducible hrpL
factor to automate the identification and cloning of HrpL-

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine