Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
JOURNAL OF BACTERIOLOGY, June 1987, p. 2424-2431 0021-9193/87/062424-08$02.00/0
 

Summary: JOURNAL OF BACTERIOLOGY, June 1987, p. 2424-2431
0021-9193/87/062424-08$02.00/0
Copyright 1987, American Society for Microbiology
Vol. 169, No. 6
Rhizobium meliloti ntrA (rpoN) Gene Is Required for Diverse
Metabolic Functions
CLIVE W. RONSON,1'2t B. TRACY NIXON,'t LISA M. ALBRIGHT,1 AND FREDERICK M. AUSUBELl*
Department of Genetics, Harvard Medical School, and Department ofMolecular Biology, Massachusetts General
Hospital, Boston, Massachusetts 02114,1 and Grasslands Division, Department of Scientific and Industrial Research,
Private Bag, Palmerston North, New Zealand2
Received 23 January 1987/Accepted 9 March 1987
We report the identification and cloning of an ntrA-like (glnF rpoN) gene ofRhizobium meliloti and show that
the R. meliloi ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation
and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with
KkebsieUla pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. melUoti ntrA
promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.
In the enteric bacteria Escherichia coli, Klebsiella pneu-
moniae, and Salmonella typhimurium, the products of the
three genes ntrA (glnF rpoN), ntrB (glnL), and ntrC (glnG)
regulate the transcriptional activation of several operons

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine