Summary: Cold RNA transcription with oligo
Aim : Transcript a RNA with an oligo.
Material :
- TRX buffer 10X
- NTP (A, G, T and C)
- DNA template
- T7 RNA
- PEG 8000
- RNAsin
- Phenol, chloroform
- G50 column.
10X Transcription buffer 1 ml Solution stock
400 mM Tris-HCl pH 7.5 400 µl from 1 M
200 mM MgCl 200 µl 1 M
20 mM Spermidine 20 µl 1 M
0.1 % 100 X-Triton 1 µl 10 %
DEPC H2O 370 µl
Store at ­20 °C.
Method :
1. Make a mix : Final concentration

Source: Abou Elela, Sherif - Département de Microbiologie et d'Infectiologie, Université de Sherbrooke

Collections: Biology and Medicine