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Molecular Ecology Notes (2003) 3, 397399 doi: 10.1046/j.1471-8286.2003.00463.x 2003 Blackwell Publishing Ltd
 

Summary: Molecular Ecology Notes (2003) 3, 397­399 doi: 10.1046/j.1471-8286.2003.00463.x
© 2003 Blackwell Publishing Ltd
Blackwell Publishing Ltd.
PRIMER NOTE
Isolation and characterization of microsatellite markers for
the seagrass Cymodocea nodosa
F. ALBERTO,* L. CORREIA,* C. BILLOT,* C. M. DUARTE and E. SERRÃO*
*CCMAR -- Center for Marine Sciences, F.C.M.A. -- University of Algarve, Gambelas, P-8005­139, Faro, Portugal, IMEDEA
(CSIC-UIB) Instituto Mediterraneo de Estudios Avanzados, C/Miquel Marques 21, 07190 Esporles, Mallorca, Spain
Abstract
In order to study the spatial patterns of genetic diversity of a clonal marine angiosperm, the
seagrass Cymodocea nodosa, microsatellite markers were obtained by screening a genomic
library enriched for the (CT) dinucleotide motif. Of 38 primer pairs defined, 15 amplified
polymorphic microsatellites and are described. These loci identified a number of alleles
ranging from two to seven, and showed expected heterozygosity ranging from 0.35 to 0.76,
when a group of 40 individuals from Cadiz Bay in Spain was analysed. Additionally, we
describe here the multiplexing conditions for 12 of these loci.
Keywords: clonal plant, Cymodocea nodosa, microsatellites, seagrass
Received 27 February 2003; revision accepted 2 April 2003
Seagrasses are marine angiosperms that reproduce

  

Source: Alberto, Filipe - Centro de Ciencias do Mar, Universidade do Algarve

 

Collections: Environmental Sciences and Ecology; Biology and Medicine