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TECHNICAL NOTE Highly polymorphic microsatellite loci for the Parsley frog

Highly polymorphic microsatellite loci for the Parsley frog
(Pelodytes punctatus): characterization and testing
for cross-species amplification
Mirjam S. van de Vliet Onno E. Diekmann
Ester T. A. Serra~o Pedro Beja
Received: 17 April 2008 / Accepted: 6 May 2008
Springer Science+Business Media B.V. 2008
Abstract A microsatellite library was developed using
genomic DNA of the Parsley frog, Pelodytes punctatus, an
amphibian species which inhabits Mediterranean temporary
pond systems. Number of alleles and heterozygosity ranged
from 10 to 25 and from 0.66 to 0.90, respectively. Cross-
species amplification was successful for 13 of the 15 devel-
oped loci for the related species, Pelodytes ibericus. The high
levels of polymorphism revealed by these loci will be
extremely useful for characterizing the population genetic
diversity and structure and to estimate levels of dispersal and
gene flow in the species P. punctatus and P. ibericus.
Keywords Pelodytes punctatus Parsley frog


Source: Alberto, Filipe - Centro de Ciencias do Mar, Universidade do Algarve


Collections: Environmental Sciences and Ecology; Biology and Medicine