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Strategies for the in vitro Evolution of Protein Function: Enzyme Evolution by Random
 

Summary: Strategies for the in vitro Evolution of Protein
Function: Enzyme Evolution by Random
Recombination of Improved Sequences
Jeffrey C. Moore, Hua-Ming Jin, Olga Kuchner and Frances H. Arnold*
Division of Chemistry and
Chemical Engineering, Mail
Code 210-41, California
Institute of Technology
Pasadena, CA 91125, USA
Sets of genes improved by directed evolution can be recombined in vitro
to produce further improvements in protein function. Recombination is
particularly useful when improved sequences are available; costs of gen-
erating such sequences, however, must be weighed against the costs of
further evolution by sequential random mutagenesis. Four genes encod-
ing para-nitrobenzyl (pNB) esterase variants exhibiting enhanced activity
were recombined in two cycles of high-Ždelity DNA shuf¯ing and
screening. Genes encoding enzymes exhibiting further improvements in
activity were analyzed in order to elucidate evolutionary processes at the
DNA level and begin to provide an experimental basis for choosing
in vitro evolution strategies and setting key parameters for recombination.

  

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

 

Collections: Chemistry; Biology and Medicine