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Plant Molecular Biology 38: 10211029, 1998. 1998 Kluwer Academic Publishers. Printed in the Netherlands.
 

Summary: Plant Molecular Biology 38: 10211029, 1998.
1998 Kluwer Academic Publishers. Printed in the Netherlands.
1021
Identification of class B and class C floral organ identity genes from rice
plants
Hong-Gyu Kang, Jong-Seong Jeon, Sichul Lee and Gynheung An
Department of Life Science, Pohang University of Science and technology, Pohang 790-784, Republic of Korea
(author for correspondence)
Received 9 February 1998; accepted in revised form 3 June 1998
Key words: ABC model, antisense, flower development, MADS box, rice, transgenic plant
Abstract
The functions of two rice MADS-box genes were studied by the loss-of-function approach. The first gene, Os-
MADS4, shows a significant homology to members in the PISTILLATA (PI) family, which is required to specify
petal and stamen identity. The second gene, OsMADS3, is highly homologous to the members in the AGAMOUS
(AG) family that is essential for the normal development of the internal two whorls, the stamen and carpel, of
the flower. These two rice MADS box cDNA clones were connected to the maize ubiquitin promoter in an
antisense orientation and the fusion molecules were introduced to rice plants by the Agrobacterium-mediated
transformation method. Transgenic plants expressing antisense OsMADS4 displayed alterations of the second and
third whorls. The second-whorl lodicules, which are equivalent to the petals of dicot plants in grasses, were altered
into palea/lemma-like organs, and the third whorl stamens were changed to carpel-like organs. Loss-of-function

  

Source: An, Gynheung - Department of Life Science, Pohang University of Science and Technology

 

Collections: Biotechnology; Biology and Medicine