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Electron energy filtering significantly improves amplitude contrast of frozen-hydrated protein at 300 kV
 

Summary: Electron energy filtering significantly improves amplitude
contrast of frozen-hydrated protein at 300 kV
Koji Yonekura a,*, Michael B. Braunfeld b
, Saori Maki-Yonekura a,c
, David A. Agard b,d
a
The W.M. Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California,
San Francisco, 1700, 4th Street, San Francisco, CA 94158-2532, USA
b
Howard Hughes Medical Institute, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA
c
Dynamic Nano Machine Project, ICORP, JST, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan
d
The W.M. Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California,
San Francisco, CA 94158-2240, USA
Received 18 April 2006; received in revised form 12 July 2006; accepted 18 July 2006
Abstract
The amplitude contrast of frozen-hydrated biological samples was measured using the bacterial flagellar filament embedded in vitre-
ous ice at an accelerating voltage of 300 kV. From the mean radial amplitude spectra of overfocused images, amplitude contrast was
estimated to be 6.9 1.9% and 2.7 1.0% of the whole contrast at the low spatial frequency range with and without energy filtering,

  

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

 

Collections: Biotechnology; Biology and Medicine