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Appl. Phys. B 80, 809816 (2005) Applied Physics B DOI: 10.1007/s00340-005-1801-x Lasers and Optics
 

Summary: Appl. Phys. B 80, 809816 (2005) Applied Physics B
DOI: 10.1007/s00340-005-1801-x Lasers and Optics
S.B. ANDERSSON Tracking a single fluorescent molecule
with a confocal microscope
Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
Received: 26 January 2005 / Revised version: 7 March 2005
Published online: date Springer-Verlag 2005
ABSTRACT We consider the problem of tracking a single flu-
orescent molecule in both two and three dimensions using a
confocal laser scanning microscope. An estimate of the posi-
tion of the molecule is generated from the measured fluores-
cence signal through the use of parameter estimation theory.
This estimate is used in a nonlinear controller designed both
to track the position of the molecule and to provide good
measurements for use in the estimation algorithm. The per-
formance of the approach is investigated through numerical
simulation for molecules undergoing diffusion and directed
transport and the capabilities of the controller relative to ex-
perimental limitations are discussed.
PACS 87.64.Tt; 87.64.Ni; 87.16.Uv

  

Source: Andersson, Sean B. - Department of Aerospace and Mechanical Engineering, Boston University

 

Collections: Engineering