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Reversible and Irreversible Effects of Chemical Fixation on the NMR Properties of Single Cells
 

Summary: Reversible and Irreversible Effects of Chemical Fixation
on the NMR Properties of Single Cells
Armin Purea1
and Andrew G. Webb2*
The effects of chemical fixation are known to alter MR param-
eters, such as relaxation times and the apparent diffusion co-
efficient (ADC) of water. It is often assumed that such changes
are reversible after samples have been reimmersed in a buffer
solution for a sufficient period of time. In this study we charac-
terize the changes associated with fixation of single Xenopus
laevis oocytes and their subsequent reimmersion in buffer. Sub-
stantial reductions in both T1 and T2 values were measured for
all compartments of the cell after fixation, with the cytoplasm
showing larger changes than the nucleus. After reimmersion in
buffer, there were small but statistically significant differences
in MR parameters between fresh and reimmersed cells. Exper-
iments with a gadolinium (Gd) contrast agent showed evidence
of irreversible changes in the permeability of cellular membranes
to small molecules. Magn Reson Med 56:927931, 2006. 2006
Wiley-Liss, Inc.

  

Source: Andrews, Anne M. - Huck Institutes of the Life Sciences, Pennsylvania State University

 

Collections: Biology and Medicine